A human neutralizing antibody against a conformational epitope shared by oligomeric SARS S1 protein.
Identifieur interne : 004009 ( Main/Exploration ); précédent : 004008; suivant : 004010A human neutralizing antibody against a conformational epitope shared by oligomeric SARS S1 protein.
Auteurs : Jinzhu Duan [République populaire de Chine] ; Xin Ji ; Jing Feng ; Wei Han ; Panhe Zhang ; Wuchun Cao ; Xueming Guo ; Cai Qi ; Dongling Yang ; Gang Jin ; Guangxia Gao ; Xiyun YanSource :
- Antiviral therapy [ 1359-6535 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Banque de peptides, Cellules Vero, Conformation des protéines, Dimérisation, Données de séquences moléculaires, Fragments d'immunoglobuline (), Fragments d'immunoglobuline (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Humains, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Séquence d'acides aminés, Tests de neutralisation, Virus du SRAS (immunologie), Épitopes (), Épitopes (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Fragments d'immunoglobuline, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS, Épitopes.
- Animaux, Banque de peptides, Cellules Vero, Conformation des protéines, Dimérisation, Données de séquences moléculaires, Fragments d'immunoglobuline, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Protéines de l'enveloppe virale, Séquence d'acides aminés, Tests de neutralisation, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (immunology), Chlorocebus aethiops, Dimerization, Epitopes (chemistry), Epitopes (immunology), Humans, Immunoglobulin Fragments (chemistry), Immunoglobulin Fragments (immunology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Molecular Sequence Data, Neutralization Tests, Peptide Library, Protein Conformation, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology).
- MESH :
- chemical , chemistry : Epitopes, Immunoglobulin Fragments, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Epitopes, Immunoglobulin Fragments, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Dimerization, Humans, Molecular Sequence Data, Neutralization Tests, Peptide Library, Protein Conformation, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
An antibody phage-display library was constructed from the B cells of convalescent severe acute respiratory syndrome (SARS) patients and screened using inactivated SARS coronavirus (CoV) virions as antigens. More than 80 positive clones were isolated from the library and one of them, scFv H12, was extensively characterized. scFv H12 bound to SARS-CoV with high affinity (equilibrium dissociation constant, Kd=73.5 nM), and neutralized SARS virions in vitro. The facts that scFv H12 bound to the SARS-S1 protein under non-reducing conditions and that it did not bind to monomeric S1 protein under reducing conditions strongly suggest that scFv H12 recognizes a conformational epitope shared by oligomeric S1 proteins. This study should aid in the manufacture of neutralizing antibody, provide a better understanding the immunological characteristics of SARS protein and facilitate the design of a SARS vaccine.
PubMed: 16518967
Affiliations:
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Le document en format XML
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last="Feng">Jing Feng</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Zhang, Panhe" sort="Zhang, Panhe" uniqKey="Zhang P" first="Panhe" last="Zhang">Panhe Zhang</name></author><author><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name></author><author><name sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name></author><author><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name></author><author><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></author><author><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name></author><author><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia 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uniqKey="Ji X" first="Xin" last="Ji">Xin Ji</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Zhang, Panhe" sort="Zhang, Panhe" uniqKey="Zhang P" first="Panhe" last="Zhang">Panhe Zhang</name></author><author><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name></author><author><name sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name></author><author><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name></author><author><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></author><author><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name></author><author><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name></author><author><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name></author></analytic><series><title level="j">Antiviral therapy</title><idno type="ISSN">1359-6535</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Chlorocebus aethiops</term><term>Dimerization</term><term>Epitopes (chemistry)</term><term>Epitopes (immunology)</term><term>Humans</term><term>Immunoglobulin Fragments (chemistry)</term><term>Immunoglobulin Fragments (immunology)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Peptide Library</term><term>Protein Conformation</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Banque de peptides</term><term>Cellules Vero</term><term>Conformation des protéines</term><term>Dimérisation</term><term>Données de séquences moléculaires</term><term>Fragments d'immunoglobuline ()</term><term>Fragments d'immunoglobuline (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Virus du SRAS (immunologie)</term><term>Épitopes ()</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Epitopes</term><term>Immunoglobulin Fragments</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Epitopes</term><term>Immunoglobulin Fragments</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments d'immunoglobuline</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Dimerization</term><term>Humans</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Peptide Library</term><term>Protein Conformation</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Banque de peptides</term><term>Cellules Vero</term><term>Conformation des protéines</term><term>Dimérisation</term><term>Données de séquences moléculaires</term><term>Fragments d'immunoglobuline</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Protéines de l'enveloppe virale</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An antibody phage-display library was constructed from the B cells of convalescent severe acute respiratory syndrome (SARS) patients and screened using inactivated SARS coronavirus (CoV) virions as antigens. More than 80 positive clones were isolated from the library and one of them, scFv H12, was extensively characterized. scFv H12 bound to SARS-CoV with high affinity (equilibrium dissociation constant, Kd=73.5 nM), and neutralized SARS virions in vitro. The facts that scFv H12 bound to the SARS-S1 protein under non-reducing conditions and that it did not bind to monomeric S1 protein under reducing conditions strongly suggest that scFv H12 recognizes a conformational epitope shared by oligomeric S1 proteins. This study should aid in the manufacture of neutralizing antibody, provide a better understanding the immunological characteristics of SARS protein and facilitate the design of a SARS vaccine.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name><name sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name><name sortKey="Ji, Xin" sort="Ji, Xin" uniqKey="Ji X" first="Xin" last="Ji">Xin Ji</name><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name><name sortKey="Zhang, Panhe" sort="Zhang, Panhe" uniqKey="Zhang P" first="Panhe" last="Zhang">Panhe Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Duan, Jinzhu" sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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